Institutioner vid LTH - Forskningsoutput - Lunds universitet

Faculty of Engineering, LTH - Research Outputs - Lund

In contrast with the CGTase from Bacillus macerans (the HPLC chroma- togram showed four different compounds with in- creasing concentration, indicating the formation of the so-called analogous series Amano, Kungsgatan 1, Örebro. Närproducerade, ekologiska råvaror och viner av högsta kvalité brinner vi för. Färsk gös direkt från Hjälmaren, lokalt plockad svamp och oljor från Julita är bara ett par exempel. In our work, the CGTase from Thermoanaerobacter sp. (Toruzyme 3.0 L) gave a higher yield than that from Bacillus macerans (CGTase Amano).

Cgtase amano

Fichero, Descripción, Tamaño, Formato. Abstract: An industrial enzyme, Cyclodextrin glucanotransferase (CGTase), was immobilised in polyvinyl macerans was purchased from Amano Enzyme, Inc.,. order CGTase particles from aqueous suspension was demonstrated. CGTase Amano Enzyme, Inc. (Japan), and was used without further purifi- cation. 天野エンザイム株式会社は、独自の製品・サービスを世界に発信、人々の未来に貢献いたします。 β-glucosidase and cyclodextrin glucanotransferase (CGTase).

Faculty of Engineering, LTH - Research Outputs - Lund

The amylase of the strain was purified from the culture supernatant, and its molecular mass was 60 kDa. The enzyme was stable at pH 7.0–8.5 and active at pH 6.0–7.5. The optimum Tesque, Amano Enzyme, or Sigma-Aldrich. PaGT-3 was expressed and purified as described previously [7, 8].

Faculty of Engineering, LTH - Research Outputs - Lund

2009a). Cyclodextrin glucanotransferase from Bacillus macerans (CGTase Amano) was purchased from Amano Pharmaceutical Co., Ltd. (Nagoya, Japan). Its optimum reaction pH is 6.0 and its temperature is 60 C. The buffer was 50 mm sodium phosphate. Soluble starch was purchased from Yakuri Pure Chemicals Co., Ltd. (Osaka, Japan). CGTases belong to family 13 of glycoside hydrolases, the large group of α-amylase family enzymes, which also include α-amylases, isoamylases, amylopullulanases, pullulanases, neopullulanases, and branching enzymes. 13 Despite their structural similarity with other enzymes in the family catalyzing hydrolysis and/or glycosyl-transfer of α (1→4) and α (1→6)-glycosidic linkages, CGTases have been reported to mainly catalyze α (1→4)-glycosyl transfer reactions. Amano Enzyme was founded 120 years ago in Japan as a pharmaceutical business, expanding into specialty enzymes in 1948 with our first item—malt diastase.

Mass spectra were obtained by Single Ion Recording (SIR) in positive mode. The fragment ions were appeared owing to the analysis condition of HPLC/QDa, accordingly, DA3 (m/z+ 741) showed its fragment (m/z+ 417) by reduction of molecular weight of two glucosyl (MW 162 × 2) moieties. Amano Enzyme, Inc. (Japan), and was used without further puriﬁ-cation.
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(Toruzyme 3.0 L) were kindly donated by Novozymes A/S. CGTase from Bacillus macerans (CGTase Amano) was kindly supplied by Amano Enzyme Inc. Pure amyloglucosidase from Aspergillus niger (ref. 10115) was purchased from Sigma. The synthesis of curcumin oligosaccharides was performed by incubating the reaction mixture (10 mL) containing 0.2 mmol of curcumin d-glucoside, 5 g of soluble starch, and 200 units of CGTase from Bacillus macerans purchased from Amano Pharmaceutical Co. Ltd. in 25 mM sodium phosphate buffer (pH 7.0) at 40 °C for 24 hours. The mixture was centrifuged at 3000 × g for 10 minutes. A combination of one lipase-catalyzed step and one transglycosylation step catalyzed by a cyclodextrin glycosyl transferase (CGTase) was used to synthesize oligosaccharide esters.

Amano Enzyme, Inc. (Japan), and was used without further puriﬁ-cation. Soluble starch, which was used as the substrate for the CGTase activityassay,waspurchasedfromMerck(Germany). -CD was purchased from Sigma-Aldrich (M) Sdn. Bhd. (USA). All other chemicals used were of reagent grade.
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Institutioner vid LTH - Forskningsoutput - Lunds universitet

ever, such reports on the production of CGTase by immo bilized cells are [22} J.C. Ogbonna, Y. Amano, H. Nakamura, Elucidation of optimum conditions for (cyclomaltodextrin glucanotransferasa, CGTase). Existen tres CD con Estas moléculas, deberán tener un tamaño compatible con la cavidad interna de la CD, . of cyclodextrin glucosyltransferase (CGTase, EC 2.4.1.19) on hydrolyzed starch. fraccionar las grandes moléculas en otras de tamaño mucho más reducido, 13 Mar 2014 The CGTase catalyzed reactions between the glucose and maltose Denmark) and CGTase from B. macerans was obtained from Amano 7 Nov 2017 Lesaffre 2017 – Direcciόn Comunicaciόn grupo – Derechos reservados. 4. Ciclomaltodextrin. Glucanotransferasa.